Acute up-regulation of brain-derived neurotrophic factor expression resulting from experimentally induced injury in the rat spinal cord

Citation
O. Ikeda et al., Acute up-regulation of brain-derived neurotrophic factor expression resulting from experimentally induced injury in the rat spinal cord, ACT NEUROP, 102(3), 2001, pp. 239-245
Citations number
60
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
ACTA NEUROPATHOLOGICA
ISSN journal
0001-6322 → ACNP
Volume
102
Issue
3
Year of publication
2001
Pages
239 - 245
Database
ISI
SICI code
0001-6322(200109)102:3<239:AUOBNF>2.0.ZU;2-Z
Abstract
Brain-derived neurotrophic factor (BDNF), a member of the nerve growth fact or family of trophic factors, has multiple functions including a role in th e promotion of neuronal survival and nerve fiber elongation in both the cen tral and the peripheral nervous systems. We assessed the expression of endo genous BDNF following an experimentally induced compression injury to the s pinal cord. Expression of BDNF mRNA was increased following the spinal cord injury; reaching maximum levels 24 h after the injury. Expression of BDNF mRNA returned to the levels observed in sham-operated control animals withi n 3 days of the injury. Using the in situ hybridization technique, we obser ved a wide distribution of BDNF expression among the different cell types i n the spinal cord, including motor and sensory neurons, and in glia cells, including astrocytes. We also observed expression of BDNF in putative macro phages and/or microglia; however, this effect was not observed until day 7 following spinal cord injury. These results suggest that BDNF is synthesize d in both neurons and astrocytes during the acute response to injury to the spinal cord, functioning in a mainly neuroprotective role. This is followe d by a later phase of expression in which BDNF is produced by macrophages a nd/or microglia, apparently functioning in a restorative capacity.