Integrated system for high-throughput protein identification using a microfabricated device coupled to capillary electrophoresis/nanoelectrospray mass spectrometry

Citation
Jj. Li et al., Integrated system for high-throughput protein identification using a microfabricated device coupled to capillary electrophoresis/nanoelectrospray mass spectrometry, PROTEOMICS, 1(8), 2001, pp. 975-986
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
PROTEOMICS
ISSN journal
1615-9853 → ACNP
Volume
1
Issue
8
Year of publication
2001
Pages
975 - 986
Database
ISI
SICI code
1615-9853(200108)1:8<975:ISFHPI>2.0.ZU;2-U
Abstract
An integrated microsystem providing rapid analyses of trace-level tryptic d igests for proteomics application is presented. This modular microsystem in cludes an autosampler and a microfabricated device comprising a sample intr oduction port and an array of separation channels together with a low dead- volume facilitating the interface to nanoelectrospray mass spectrometry. Se quential injection and separation of peptide standards and tryptic digests was achieved with a throughput of up to 30 samples per hour with less than 3% sample carryover. Replicate injections of peptide mixtures indicated tha t reproducibility of migration time was typically better than 2.3% relative standard deviation (RSD) whereas RSD values of 3.7-11.8% were observed on peak height. Mass spectral detection of submicromolar protein digests (< 7 femtomoles/injection) was achieved using a quadrupole/time of flight instru ment in less than 2 min/per sample with peak widths of 1.8-7.0 s. The analy tical potential of this integrated device for the identification of gel iso lated proteins from Neisseria meningitidis immunotype L3 has been demonstra ted using both peptide mass-fingerprint database searching and on-line tand em mass spectrometry.