Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis

Citation
S. Iuchi et al., Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis, PLANT J, 27(4), 2001, pp. 325-333
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT JOURNAL
ISSN journal
0960-7412 → ACNP
Volume
27
Issue
4
Year of publication
2001
Pages
325 - 333
Database
ISI
SICI code
0960-7412(200108)27:4<325:RODTBG>2.0.ZU;2-H
Abstract
Abscisic acid (ABA), a plant hormone, is involved in responses to environme ntal stresses such as drought and high salinity, and is required for stress tolerance. ABA is synthesized de novo in response to dehydration. 9-cis-ep oxycarotenoid dioxygenase (NCED) is thought to be a key enzyme in ABA biosy nthesis. Here we demonstrate that the expression of an NCED gene of Arabido psis, AtNCED3, is induced by drought stress and controls the level of endog enous ABA under drought-stressed conditions. Overexpression of AtNCED3 in t ransgenic Arabidopsis caused an increase in endogenous ABA level, and promo ted transcription of drought- and ABA-inducible genes. Plants overexpressin g AtNCED3 showed a reduction in transpiration rate from leaves and an impro vement in drought tolerance. By contrast, antisense suppression and disrupt ion of AtNCED3 gave a drought-sensitive phenotype. These results indicate t hat the expression of AtNCED3 plays a key role in ABA biosynthesis under dr ought-stressed conditions in Arabidopsis. We improved drought tolerance by gene manipulation of AtNCED3 causing the accumulation of endogenous ABA.