Soluble products from Eikenella corrodens stimulate oral epithelial cells to induce inflammatory mediators

Citation
H. Yumoto et al., Soluble products from Eikenella corrodens stimulate oral epithelial cells to induce inflammatory mediators, ORAL MICROB, 16(5), 2001, pp. 296-305
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ORAL MICROBIOLOGY AND IMMUNOLOGY
ISSN journal
0902-0055 → ACNP
Volume
16
Issue
5
Year of publication
2001
Pages
296 - 305
Database
ISI
SICI code
0902-0055(200110)16:5<296:SPFECS>2.0.ZU;2-Y
Abstract
In the inflammatory response elicited by bacterial colonization in periodon tal pockets, pocket epithelial cells not only serve as a barrier to isolate the pocket microenvironment from external stimuli but also regulate the fu nctions of neighboring cells including fibroblasts and inflammatory cells. To elucidate this mechanism, we characterized the effects of periodontopath ic bacterium Eikenella corrodens 1073 components on the production of some inflammatory mediators in a human oral epithelial cell line (KB). In enzyme -linked immunosorbent assay (ELISA), the E. corrodens supernatant induced i nterleukin-6 (IL-6), IL-8 and prostaglandin E-2 but not interferon-gamma (I FN-gamma) production by KB cells. After incubation with E. corrodens supern atant, YB cells showed a marked increase in the levels of IL-6, IL-8 and PG G/H synthase (cyclooxygenase)-2, but not IFN-gamma, gene expression by rev erse-transcriptase polymerase chain reaction. All these E. corrodens produc ts responsible for production of these inflammatory mediators resisted free zing and boiling and were present in a 10-kDa filtrate. These results imply that these soluble small-molecular-mass products from E. Corrodens stimula te various inflammatory mediator productions by human oral epithelial cells and may play a role in the initiation of periodontal inflammation and subs equently perpetuate the inflammatory response during chronic infection.