Limitted degradation of alpha-actinin in the ordinary muscle of cultured red sea bream during storage in ice

Citation
K. Tachibana et al., Limitted degradation of alpha-actinin in the ordinary muscle of cultured red sea bream during storage in ice, NIP SUIS G, 67(4), 2001, pp. 723-727
Citations number
8
Language
GIAPPONESE
art.tipo
Article
Categorie Soggetti
Aquatic Sciences
Journal title
NIPPON SUISAN GAKKAISHI
ISSN journal
0021-5392 → ACNP
Volume
67
Issue
4
Year of publication
2001
Pages
723 - 727
Database
ISI
SICI code
0021-5392(200107)67:4<723:LDOAIT>2.0.ZU;2-J
Abstract
Anti-alpha -actinin IgG was prepared from rat serum immunized with purified alpha -actinin in the ordinary muscle of red sea bream. Anti-alpha -actini n IgG showed immune reactivity against not only alpha -actinin in ordinary muscle but also papain-digests of the alpha -actinin. According to immunoel ectron microscopic method, alpha -actinin was detected on Z line of ordinar y muscle cells and non-specific reactions were not detected on another orga nelles. The changes of alpha -actinin in the ordinary muscle of cultured re d sea bream during storage in ice were examined by SDS-PAGE followed by its immunoblotting. It was found that the limited digestion of alpha -actinin in ordinary muscle of cultured red sea bream started on day one in ice, the n, native alpha -actinin was not detected on day 5 and 7. These results sug gested that the weakening of Z-disks in ordinary muscle was caused by the d isappearance of alpha -actinin and resulted in the softness of the flesh te xture of cultured red sea bream.