Detection of Ebola viral antigen by enzyme-linked immunosorbent assay using a novel monoclonal antibody to nucleoprotein

Citation
M. Niikura et al., Detection of Ebola viral antigen by enzyme-linked immunosorbent assay using a novel monoclonal antibody to nucleoprotein, J CLIN MICR, 39(9), 2001, pp. 3267-3271
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
0095-1137 → ACNP
Volume
39
Issue
9
Year of publication
2001
Pages
3267 - 3271
Database
ISI
SICI code
0095-1137(200109)39:9<3267:DOEVAB>2.0.ZU;2-T
Abstract
With the increase in international traffic, the risk of introducing rare bu t severe infectious diseases like Ebola hemorrhagic fever is increasing all over the world. However, the system for the diagnosis of Ebola virus infec tion is available in a limited number of countries. In the present study, w e developed an Ebola virus antigen-detection enzyme-linked immunosorbent as say (ELISA) system using a novel monoclonal antibody (MAb) to the nucleopro tein (NP). This antibody recognized an epitope defined by a 26-amino-acid s tretch near the C terminus of NP. In a sandwich ELISA system with the MAb, as little as 30 ng of purified recombinant NP (rNP) was detected. Although this MAb was prepared by immunization with rNP of subtype Zaire, it also re acted to the corresponding region of NP derived from the Reston and Sudan s ubtypes. These results suggest that our ELISA system should work with three of four Ebola subtypes. Furthermore, our ELISA system detected the NP in s ubtype Reston-infected monkey specimens, while the background level in noni nfected specimens was very low, suggesting the usefulness of the ELISA for laboratory diagnosis with clinical specimens.