Reliable detection of respiratory syncytial virus infection in children for adequate hospital infection control management

Citation
S. Abels et al., Reliable detection of respiratory syncytial virus infection in children for adequate hospital infection control management, J CLIN MICR, 39(9), 2001, pp. 3135-3139
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
0095-1137 → ACNP
Volume
39
Issue
9
Year of publication
2001
Pages
3135 - 3139
Database
ISI
SICI code
0095-1137(200109)39:9<3135:RDORSV>2.0.ZU;2-L
Abstract
By using a rapid test for respiratory syncytial virus (RSV) detection (Abbo tt TestPack RSV), a number of patients were observed, showing repeatedly po sitive results over a period of up to 10 weeks. A prospective study was ini tiated to compare the rapid test with an antigen capture enzyme immunoassay (EIA) and a nested reverse transcriptase PCR (RT-PCR) protocol for detecti on of RSV serotypes A and B. Only respiratory samples from children exhibit ing the prolonged presence of RSV (greater than or equal to5 days) as deter mined by the rapid test were considered. A total of 134 specimens from 24 c hildren was investigated by antigen capture EIA and nested RT-PCR. Using RT -PCR as the reference method, we determined the RSV rapid test to have a sp ecificity of 63% and a sensitivity of 66% and the antigen capture EIA to ha ve a specificity of 96% and a sensitivity of 69% for acute-phase samples an d the homologous virus serotype A. In 7 (29%) of 24 patients, the positive results of the RSV rapid test could not be confirmed by either nested RT-PC R or antigen capture EU. In these seven patients a variety of other respira tory viruses were detected. For general screening the RSV rapid test was fo und to be a reasonable tool to get quick results. However, its lack of spec ificity in some patients requires confirmation by additional tests to rule out false-positive results and/or detection of other respiratory viruses.