LightCycler multiplex PCR for the laboratory diagnosis of common viral infections of the central nervous system

Citation
Sj. Read et al., LightCycler multiplex PCR for the laboratory diagnosis of common viral infections of the central nervous system, J CLIN MICR, 39(9), 2001, pp. 3056-3059
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
0095-1137 → ACNP
Volume
39
Issue
9
Year of publication
2001
Pages
3056 - 3059
Database
ISI
SICI code
0095-1137(200109)39:9<3056:LMPFTL>2.0.ZU;2-L
Abstract
A conventional multiplex PCR assay that detects herpes simplex virus type 1 (HSV-1), HSV-2, varicella-zoster virus, and enteroviruses for the diagnosi s of central nervous system infections was modified to be performed using t he LightCycler system. The sensitivity of detection of each of the viruses using the LightCycler assay was compared to that of the conventional assay using external quality assessment material. The assays had equivalent sensi tivities, but the LightCycler assay was more rapid, reduced the risk of con tamination, and used an amplicon detection format that demonstrated greater discrimination than a get electrophoresis method.