Identification of strains of Alcaligenes and Agrobacterium by a polyphasicapproach

Citation
D. Clermont et al., Identification of strains of Alcaligenes and Agrobacterium by a polyphasicapproach, J CLIN MICR, 39(9), 2001, pp. 3104-3109
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
0095-1137 → ACNP
Volume
39
Issue
9
Year of publication
2001
Pages
3104 - 3109
Database
ISI
SICI code
0095-1137(200109)39:9<3104:IOSOAA>2.0.ZU;2-J
Abstract
The number of stable discriminant biochemical characters is limited in the genera Alcaligenes and Agrobacterium, whose species are consequently diffic ult to distinguish from one another by conventional tests. Moreover, genomi c studies have recently drastically modified the nomenclature of these gene ra; for example, Alcaligenes xylosaxidans was transferred to the genus Achr omobacter in 1998. Twenty-five strains of Achromobacter xylosoxidans, three strains of an Agrobacterium sp., five strains of an Alcaligenes sp., and f our unnamed strains belonging to the Centers for Disease Control and Preven tion group IVc-2 were examined. These strains were characterized by convent ional tests, including biochemical tests. The assimilation of 99 carbohydra tes, organic acids, and amino acids was studied by using Biotype-100 strips , and rRNA gene restriction patterns were obtained with the automated Ribop rinter microbial characterization system after cleavage of total DNA with E coRI or PstI restriction endonuclease. This polyphasic approach allowed the two subspecies of A. xylosoxidans to be clearly separated. Relationships b etween five strains and the Ralstonia paucula type strain were demonstrated . Likewise, three strains were found to be related to the Ochrobactrum anth ropi type strain. We showed that substrate assimilation tests and automated ribotyping provide a simple, rapid, and reliable means of identifying A. x ylosoxidans subspecies and that these two methods can be used as alternativ e methods to characterize unidentified strains rapidly when discriminant bi ochemical characters are missing.