Rapid and accurate identification of coagulase-negative staphylococci by real-time PCR

Citation
Kj. Edwards et al., Rapid and accurate identification of coagulase-negative staphylococci by real-time PCR, J CLIN MICR, 39(9), 2001, pp. 3047-3051
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
0095-1137 → ACNP
Volume
39
Issue
9
Year of publication
2001
Pages
3047 - 3051
Database
ISI
SICI code
0095-1137(200109)39:9<3047:RAAIOC>2.0.ZU;2-H
Abstract
Biprobe identification assays based on real-time PCR were designed for 15 s pecies of coagulase-negative staphylococci (CNS). Three sets of primers and four biprobes were designed from two variable regions of the 16S rRNA gene . An identification scheme was developed based on the pattern of melting pe aks observed with the four biprobes that had been tested on 24 type strains . This scheme was then tested on 100 previously identified clinical isolate s and 42 blindly tested isolates. For 125 of the 142 clinical isolates ther e was a perfect correlation between the biprobe identification and the resu lt of the ID 32 Staph phenotypic tests and PCR. For 12 of the other isolate s a 300-bp portion of the 16S rRNA gene was sequenced to determine identity . The remaining five isolates could not be fully identified. LightCycler re al-time PCR allowed rapid and accurate identification of the important CNS implicated in infection.