A transformation system for an ectomycorrhizal basidiomycete, Lyophyllum shimeji

Citation
T. Saito et al., A transformation system for an ectomycorrhizal basidiomycete, Lyophyllum shimeji, BIOS BIOT B, 65(8), 2001, pp. 1928-1931
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
0916-8451 → ACNP
Volume
65
Issue
8
Year of publication
2001
Pages
1928 - 1931
Database
ISI
SICI code
0916-8451(200108)65:8<1928:ATSFAE>2.0.ZU;2-5
Abstract
A transformation vector, pLS-hph, was constructed with the promoter and ter minator of the glyceraldehyde-3-phosphate dehydrogenase (GPD) gene derived from an ectomycorrhizal basidiomycete, Lyophyllum shimeji, and with the hyg romycin B (HmB) phosphotransferase (hph) gene from Escherichia coli. This v ector was introduced into protoplasts of L. shimeji and 3.4 transformants p er mug plasmid DNA were obtained. In most of the transformants, multiple co pies of the vector were integrated into the genomic DNA. The results indica te that pLS-hph is a useful vector for L. shimeji.