A transformation vector, pLS-hph, was constructed with the promoter and ter
minator of the glyceraldehyde-3-phosphate dehydrogenase (GPD) gene derived
from an ectomycorrhizal basidiomycete, Lyophyllum shimeji, and with the hyg
romycin B (HmB) phosphotransferase (hph) gene from Escherichia coli. This v
ector was introduced into protoplasts of L. shimeji and 3.4 transformants p
er mug plasmid DNA were obtained. In most of the transformants, multiple co
pies of the vector were integrated into the genomic DNA. The results indica
te that pLS-hph is a useful vector for L. shimeji.