Strand displacement recognition of mixed adenine-cytosine sequences in double stranded DNA by thymine-guanine PNA (peptide nucleic acid)

Citation
Pe. Nielsen et M. Egholm, Strand displacement recognition of mixed adenine-cytosine sequences in double stranded DNA by thymine-guanine PNA (peptide nucleic acid), BIO MED CH, 9(9), 2001, pp. 2429-2434
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY
ISSN journal
0968-0896 → ACNP
Volume
9
Issue
9
Year of publication
2001
Pages
2429 - 2434
Database
ISI
SICI code
0968-0896(200109)9:9<2429:SDROMA>2.0.ZU;2-4
Abstract
Mixed pyrimidine-purine peptide nucleic acids (PNAs) composed of thymines a nd guanines are shown to form a PNA(2)-DNA triplex with Watson-Crick comple mentary adenine-cytosine oligonucleotides and to bind complementary adenine -cytosine targets in double stranded DNA by helix invasion. These results f or the first time demonstrate binding of an unmodified PNA oligomer to a mi xed pyrimidine-purine target in double stranded DNA and illustrate a novel binding mode of PNA. (C) 2001 Published by Elsevier Science Ltd.