Pe. Nielsen et M. Egholm, Strand displacement recognition of mixed adenine-cytosine sequences in double stranded DNA by thymine-guanine PNA (peptide nucleic acid), BIO MED CH, 9(9), 2001, pp. 2429-2434
Mixed pyrimidine-purine peptide nucleic acids (PNAs) composed of thymines a
nd guanines are shown to form a PNA(2)-DNA triplex with Watson-Crick comple
mentary adenine-cytosine oligonucleotides and to bind complementary adenine
-cytosine targets in double stranded DNA by helix invasion. These results f
or the first time demonstrate binding of an unmodified PNA oligomer to a mi
xed pyrimidine-purine target in double stranded DNA and illustrate a novel
binding mode of PNA. (C) 2001 Published by Elsevier Science Ltd.