Alpha-synuclein expression is up-regulated in NTera2 cells during neuronaldifferentiation but unaffected by exposure to cytokines and neurotrophic factors

Satoh, JI Kuroda, Y

Ji. Satoh et Y. Kuroda, Alpha-synuclein expression is up-regulated in NTera2 cells during neuronaldifferentiation but unaffected by exposure to cytokines and neurotrophic factors, PARKINS R D, 8(1), 2001, pp. 7-17

37

INGLESE

Article

Neurology

PARKINSONISM & RELATED DISORDERS

1353-8020 → ACNP

8

1

2001

7 - 17

ISI

1353-8020(200109)8:1<7:AEIUIN>2.0.ZU;2-D

Increasing evidence has indicated that proinflammatory cytokines such as TN F-alpha and IL-1 beta, produced by activated microglia and astrocytes, play a key role in progressive degeneration of the nigrostriatal dopaminergic n eurons in Parkinson's disease (PD). Since alpha -synuclein is a major compo nent of Lewy bodies in PD brains, we studied the constitutive and cytokine/ neurotrophic factor-regulated expression of alpha -synuclein in cultured hu man neurons by Northern blot and Western blot analyses. The constitutive ex pression of alpha -synuclein mRNA was identified in a variety of human neur al and non-neural cell lines. The levels of alpha -synuclein expression wer e elevated markedly in NTera2 teratocarcinoma cells following retinoic acid -induced neuronal differentiation, accompanied with an increased expression of synphilin-1, while they were unaltered in NTera2-derived differentiated neurons by exposure to TNF-alpha, IL-1 beta, BDNF or GDNF. These results i ndicate that a-synuclein expression in human neurons is up-regulated during differentiation, but is unaffected by a panel of cytokines and neurotrophi c factors which are supposed to be involved in the nigral neuronal death an d survival. (C) 2001 Elsevier Science Ltd. All rights reserved.