Binding of the S7 protein with fragment 926-986/1219-1393 of the 16S rRNA as a key step in the assembly of the small subunit of prokaryotic ribosomes

Citation
Ti. Rassokhin et al., Binding of the S7 protein with fragment 926-986/1219-1393 of the 16S rRNA as a key step in the assembly of the small subunit of prokaryotic ribosomes, MOL BIOL, 35(4), 2001, pp. 527-535
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR BIOLOGY
ISSN journal
0026-8933 → ACNP
Volume
35
Issue
4
Year of publication
2001
Pages
527 - 535
Database
ISI
SICI code
0026-8933(200107/08)35:4<527:BOTSPW>2.0.ZU;2-P
Abstract
Both structural and thermodynamic studies are necessary to understand the r ibosome assembly. An initial step was made in studying the interaction betw een a 16S rRNA fragment and S7, a key protein in assembling the prokaryotic ribosome small subunit. The apparent dissociation constant was obtained fo r complexes of recombinant Escherichia coli and Thermus thermophilus S7 wit h a fragment of the 3 ' domain of the E. coli 16S rRNA. Both proteins showe d high rRNA-binding activity, which was not observed earlier. Since RNA and proteins are conformationally labile, their folding must be considered to correctly describe the RNA-protein interactions.