Overexpression of NAD(P)H : quinone oxidoreductase 1 in human reproductivesystem

Citation
F. Zappa et al., Overexpression of NAD(P)H : quinone oxidoreductase 1 in human reproductivesystem, J HIST CYTO, 49(9), 2001, pp. 1187-1188
Citations number
7
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
ISSN journal
0022-1554 → ACNP
Volume
49
Issue
9
Year of publication
2001
Pages
1187 - 1188
Database
ISI
SICI code
0022-1554(200109)49:9<1187:OON:QO>2.0.ZU;2-W
Abstract
NAD(P)H:quinone oxidoreductase 1 (NQO1; DT-diaphorase; DTD) is a two-electr on reductase that efficiently bioactivates compounds of the quinone family, such as mitomycin C. The observation that DTD is overexpressed in many can cerous tissues compared to normal tissues has provided us with a potentiall y selective target that can be exploited in the design of novel anticancer agents. Because of the relative lack of information on the cell-specific ex pression of DTD, the purpose of this study was to perform a body mapping of its normal distribution. Tissue samples from various components of the hum an reproductive system were analyzed by immunohistochemistry. We found stro ng expression of this enzyme in testicular stromal cells (Leydig cells) and in the epithelium of epididymis, ductuli efferentes, and Fallopian tube. T hese results suggest that DTD-bioactivated quinones could be responsible fo r a selective toxicity on these components of the reproductive system and c ause clinical problems due to testosterone deficiency and infertility. This observation needs to be investigated in preclinical evaluation of new anti cancer quinones and in patients treated with these compounds.