A novel method using baculovirus-mediated gene transfer for production of recombinant adeno-associated virus vectors

Citation
K. Sollerbrant et al., A novel method using baculovirus-mediated gene transfer for production of recombinant adeno-associated virus vectors, J GEN VIROL, 82, 2001, pp. 2051-2060
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF GENERAL VIROLOGY
ISSN journal
0022-1317 → ACNP
Volume
82
Year of publication
2001
Part
9
Pages
2051 - 2060
Database
ISI
SICI code
0022-1317(200109)82:<2051:ANMUBG>2.0.ZU;2-5
Abstract
The baculovirus Autographa californica multiple nucleopolyhedrosis virus ca uses non-productive infection in mammalian cells. Recombinant baculovirus t herefore has the capability to transfer and express heterologous genes in t hese cells if a mammalian promoter governs the gene of interest. We have in vestigated the possibility of using baculovirus as a tool to produce recomb inant adenoassociated virus (rAAV). AAV has become increasingly popular as a vector for gene therapy and functional genomics efforts, although its use is hampered by the lack of a simple and efficient vector production method . We show here that co-infection of mammalian producer cells with three vir uses - a baculovirus containing the reporter gene flanked by AAV ITRs, a ba culovirus expressing the AAV rep gene and a helper adenovirus expressing th e AAV cap gene - produces infectious rAAV particles. This baculovirus-based chimeric vector method may in future improve large-scale rAAV vector prepa rations and circumvent present-day problems associated with rAAV production .