Chromosome instability in the murine melanoma cell line K-1735 is due to drug-specific mechanisms

Citation
Ma. Nemeth et al., Chromosome instability in the murine melanoma cell line K-1735 is due to drug-specific mechanisms, GENET MOL B, 23(4), 2000, pp. 763-769
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENETICS AND MOLECULAR BIOLOGY
ISSN journal
1415-4757 → ACNP
Volume
23
Issue
4
Year of publication
2000
Pages
763 - 769
Database
ISI
SICI code
1415-4757(200012)23:4<763:CIITMM>2.0.ZU;2-J
Abstract
The purpose of the present study was to investigate chromosomal instability and DNA repair by exposing clones from the murine melanoma cell line K-173 5 to the radiomimetic drug bleomycin and to the DNA polymerase a inhibitor aphidicolin. Results from previous experiments conducted with human lymphoc ytes have suggested synergistic chromosomal damage after simultaneous expos ure to these two agents. However, in the murine cell line studied here, the re was no direct correlation between the effects of these two agents. Indee d, the extensive variation in the responses to aphidicolin and bleomycin su ggested different mechanisms for the repair of bleomycin-induced DNA damage by the clones. Evaluation of the unexplained propensity of some bleomycin- treated metaphase cells to disintegrate suggested that this phenomenon was most likely the result of a direct action of bleomycin, rather than a poten tial manifestation of tumor cell instability.