Direct electrochemical determination of pyruvate in human sweat by capillary zone electrophoresis

Citation
Ml. Jin et al., Direct electrochemical determination of pyruvate in human sweat by capillary zone electrophoresis, ELECTROPHOR, 22(13), 2001, pp. 2793-2796
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
0173-0835 → ACNP
Volume
22
Issue
13
Year of publication
2001
Pages
2793 - 2796
Database
ISI
SICI code
0173-0835(200108)22:13<2793:DEDOPI>2.0.ZU;2-9
Abstract
Capillary zone electrophoresis was employed for the determination of pyruva te in human sweat using electrochemical detection with a carbon fiber micro disk bundle electrode at a constant potential of 1.60 V vs. saturated calom el electrode. The optimum separation conditions are 3.6 x 10(-3) mol/L Na2H PO4-1.4 x 10(-3) mol/L NaH2PO4 (pH 7.2) for the buffer solution, and 18 kV for the separation voltage, The limits of detection of pyruvate are 8.0 x 1 0(-6) mol/L or 24 fmol (S/N = 3) for the injection voltage of 6 kV and the injection time of 10 s. The relative standard deviation is 2.0% for the mig ration time and 5.7% for the electrophoretic peak current. The method was a pplied to determining pyruvate in human sweat.