Monitoring diclofenac sodium in single human erythrocytes introduced by electroporation using capillary zone electrophoresis with electrochemical detection

Dong, Q Jin, WR

Q. Dong et Wr. Jin, Monitoring diclofenac sodium in single human erythrocytes introduced by electroporation using capillary zone electrophoresis with electrochemical detection, ELECTROPHOR, 22(13), 2001, pp. 2786-2792

31

INGLESE

Article

Chemistry & Analysis

ELECTROPHORESIS

0173-0835 → ACNP

22

13

2001

2786 - 2792

ISI

0173-0835(200108)22:13<2786:MDSISH>2.0.ZU;2-6

A method for determination of the drug diclofenac sodium introduced into in dividual human erythrocytes by electroporation using capillary zone electro phoresis with electrochemical detection at a carbon fiber array microelectr ode was developed. In this method, the whole cell was injected into the sep aration capillary by electromigration. Cell lysis was accomplished by injec ting a plug of the separation buffer (1.25 x 10(-2) mol/L Na2B4O7-3.13 x 10 (-3) mol/L NaOH). The optimum conditions of separation and detection were 2 0 kV for the separation voltage and 1.0 V for the detection potential. The concentration of diclofenac sodium in the single cells was quantified by a calibration curve. The mean concentration of diclofenac sodium introduced i nto the cell was 4.21 mu mol/L. The relative standard deviation of the conc entration of diclofenac sodium introduced into ten cells is 10%.