Monitoring diclofenac sodium in single human erythrocytes introduced by electroporation using capillary zone electrophoresis with electrochemical detection

Authors
Citation
Q. Dong et Wr. Jin, Monitoring diclofenac sodium in single human erythrocytes introduced by electroporation using capillary zone electrophoresis with electrochemical detection, ELECTROPHOR, 22(13), 2001, pp. 2786-2792
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
0173-0835 → ACNP
Volume
22
Issue
13
Year of publication
2001
Pages
2786 - 2792
Database
ISI
SICI code
0173-0835(200108)22:13<2786:MDSISH>2.0.ZU;2-6
Abstract
A method for determination of the drug diclofenac sodium introduced into in dividual human erythrocytes by electroporation using capillary zone electro phoresis with electrochemical detection at a carbon fiber array microelectr ode was developed. In this method, the whole cell was injected into the sep aration capillary by electromigration. Cell lysis was accomplished by injec ting a plug of the separation buffer (1.25 x 10(-2) mol/L Na2B4O7-3.13 x 10 (-3) mol/L NaOH). The optimum conditions of separation and detection were 2 0 kV for the separation voltage and 1.0 V for the detection potential. The concentration of diclofenac sodium in the single cells was quantified by a calibration curve. The mean concentration of diclofenac sodium introduced i nto the cell was 4.21 mu mol/L. The relative standard deviation of the conc entration of diclofenac sodium introduced into ten cells is 10%.