Inhibition of itch-scratch response by fruits of Cnidium monnieri in mice

Citation
P. Basnet et al., Inhibition of itch-scratch response by fruits of Cnidium monnieri in mice, BIOL PHAR B, 24(9), 2001, pp. 1012-1015
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN journal
0918-6158 → ACNP
Volume
24
Issue
9
Year of publication
2001
Pages
1012 - 1015
Database
ISI
SICI code
0918-6158(200109)24:9<1012:IOIRBF>2.0.ZU;2-S
Abstract
We previously screened the anti-itching activities of 33 herbal medicines i n substance P (SP)-induced itching model mice. One of the most potent antip ruritogenic extracts, the methanol extract of fruits of Chidium monnieri (C nidii Fructus) was studied further. The chloroform-soluble fraction of the methanol extract markedly inhibited SP-induced scratching. Among 10 subfrac tions of the chloroform-soluble fraction, the CS-3 fraction had the most po tent inhibitory effect on scratching. Each of 3 subfractions of CS-3 showed significant anti-scratching activities. However, inhibitory potencies were not different among the three and weaker than that of CS-3 itself at a sam e dose. These 3 subfractions of CS-3 mainly contained xanthotoxin, isopimpi nellin, bergapten, imperatorin and osthol. Single administration of osthol did not inhibit SP-induced scratching, and imperatorin very weakly subsided scratching. These results suggest that the strong antipruritic. action was focused on the CS-3 fraction of the C. monnieri methanol extract, and it m ight result from the combined effects of these coumarin derivatives, or by undetermined minor compounds.