Effect of IL-1 beta and IL-6 on oxytocin secretion in human uterine smoothmuscle cells

Citation
U. Friebe-hoffmann et al., Effect of IL-1 beta and IL-6 on oxytocin secretion in human uterine smoothmuscle cells, AM J REPROD, 46(3), 2001, pp. 226-231
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
ISSN journal
1046-7408 → ACNP
Volume
46
Issue
3
Year of publication
2001
Pages
226 - 231
Database
ISI
SICI code
1046-7408(200109)46:3<226:EOIBAI>2.0.ZU;2-5
Abstract
PROBLEM: Infection-mediated preterm labor results in the production of infl ammatory cytokines, including interleukin-1 beta (IL-1 beta) and interleuki n-6 (IL-6). Oxytocin (OT) plays a key role in the process of labor. This st udy investigates the effect of IL-1 beta and IL-6 on intra-and extracellula r OT in human smooth muscle cells and evaluates IL-1 beta induced changes i n IL-6 production. METHOD OF STUDY: Primary cultures of human myometrium, obtained from term p regnant women (n = 7) were incubated with either IL-1 beta or IL-6 for 0-24 hr. Intra- and extracellular OT peptide concentrations were measured by ra dioimmunoassay and IL-6 mRNA and protein were evaluated by reverse transcri ption polymerase chain reaction and enzyme-linked immunosorbent assay. RESULTS: Both, IL-1 beta and IL-6 led to an increase in OT secretion, which was accompanied by a reduction of the intracellular OT peptide pool. IL-1 beta significantly induced IL-6 mRNA expression and protein secretion, whic h did not further enhance IL-1 beta induced OT secretion. CONCLUSIONS: The induction of OT secretion by proinflammatory cytokines in human myometrium in vitro, supports the concept of a thus regulated infecti on-triggered preterm labor process in vivo.