Circulating human antisperm antibodies recognize prostasomes

Citation
C. Allegrucci et al., Circulating human antisperm antibodies recognize prostasomes, AM J REPROD, 46(3), 2001, pp. 211-219
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
ISSN journal
1046-7408 → ACNP
Volume
46
Issue
3
Year of publication
2001
Pages
211 - 219
Database
ISI
SICI code
1046-7408(200109)46:3<211:CHAARP>2.0.ZU;2-9
Abstract
PROBLEM: The presence of naturally occurring antisperm antibodies (ASA) is a well-known cause of infertility in men and women, but the antigens for th ese antibodies are usually poorly characterized. Prostasomes, organelles se creted by human prostatic acinar cells and expelled into the seminal plasma at ejaculation, can adhere to sperm cells. Thus, we have examined whether prostasomes could be an antigen for ASA. METHOD OF STUDY: We have studied the reactivity of chicken antiprostasome a ntibodies with sperm cells in an agglutination test and conversely the reac tivity of serum positive for ASA from 20 infertile patients, with spermatoz oa using flow cytometry and with purified prostasomes using enzyme-linked i mmunosorbent assay. RESULTS: The chicken antiprostasome antibody caused agglutination of sperm cells similarly to the agglutination observed with patients' sera. All of t hese patients' sera contained IgG antibodies against prostasomes. CONCLUSIONS: The high percentage of patients with antiprostasome antibodies in this study shows that prostasomes could be one of the major targets for ASA.