Paracrine action of keratinocyte growth factor (KGF) during ruminant mammogenesis

Citation
Rc. Hovey et al., Paracrine action of keratinocyte growth factor (KGF) during ruminant mammogenesis, MOL C ENDOC, 181(1-2), 2001, pp. 47-56
Citations number
50
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Endocrinology, Nutrition & Metabolism
Journal title
MOLECULAR AND CELLULAR ENDOCRINOLOGY
ISSN journal
0303-7207 → ACNP
Volume
181
Issue
1-2
Year of publication
2001
Pages
47 - 56
Database
ISI
SICI code
0303-7207(20010705)181:1-2<47:PAOKGF>2.0.ZU;2-R
Abstract
Keratinocyte growth factor (KGF) is a stroma-derived mitogen mediating epit helial-stromal interactions. We investigated the role of KGF during epithel ial-stromal interactions accompanying ruminant marnmogenesis. Target-specif icity of KGF was demonstrated in that KGF-stimulated proliferation of bovin e mammary epithelial, but not ovine mammary stromal cells. Consistent with a paracrine function, 4.6, 2.4, 1.5 and 0.9 kb mRNA transcripts were expres sed by bovine stromal, but not epithelial cells. Within the ovine mammary g land, 2.4 and 1.5 kb KGF mRNAs were expressed in the fat pad while only the 2.4 kb transcript was transcribed in parenchyma. The abundance of KGF mRNA was greater in the extra-parenchymal fat pad than in the contralateral epi thelium-free fat pad prior to puberty, and was less in parenchyma than in t he intact or epithelium-free fat pads. Ovariectomy tended to increase KGF t ranscription while estrogen reduced expression. Of several tissues, mammary parenchyma expressed a 2.4 kb mRNA while adipose tissues expressed a 1.5 k b transcript. These results demonstrate local and systemic regulation of KG F transcription and support a paracrine role for KGF during ruminant mammog enesis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.