Dried zinc-containing yeasts (5 g zinc / 100 g yeast) were rehydrated at 20
% solids and subjected to autolysis at 50 degreesC for 24 h. Either of the
following enzymes, glucoamylase, mannanase, beta -1, 3 glucanase, fungal a
cid protease, validase FP or papain was added to promote lysis. Autolysis y
ields, recovery of zinc in the yeast extract (YE) as well as composition of
the YE (nitrogen, sugars) were determined. In the absence of added enzymes
, autolysis yield with fresh Bakers' yeast was 46 %, while that with the re
hydrated zinc-enriched yeasts was 21 %. All adjunct enzymes increased autol
ysis yields (up to 41 %) from the rehydrated zinc-enriched yeast. The fract
ion of the zinc that was recovered in the YE ranged from 64 to 81 % of the
total amount originally present in the yeast, depending on the enzymes used
(beta -1, 3 glucanase and validase FP gave best results). Yeast extracts o
btained from glucanase had higher contents of glucose, but lower contents o
f a-amino nitrogen than those produced with the proteases. The use of enzym
es to obtain YE from rehydrated zinc-containing yeasts generated YE having
15 g of zinc/100 g of yeast extract.