Removal of dissolved brown algal phlorotannins using insoluble polyvinylpolypyrrolidone (PVPP)

Authors
Citation
Gb. Toth et H. Pavia, Removal of dissolved brown algal phlorotannins using insoluble polyvinylpolypyrrolidone (PVPP), J CHEM ECOL, 27(9), 2001, pp. 1899-1910
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Environment/Ecology
Journal title
JOURNAL OF CHEMICAL ECOLOGY
ISSN journal
0098-0331 → ACNP
Volume
27
Issue
9
Year of publication
2001
Pages
1899 - 1910
Database
ISI
SICI code
0098-0331(200109)27:9<1899:RODBAP>2.0.ZU;2-3
Abstract
Tannins, a large and diverse group of phenolic secondary metabolites, are c ommon in terrestrial plants and marine brown algae. It is sometimes desirab le to remove the tannins from plant or algal extracts. e.g., when isolating enzymes and nucleic acids, when using certain colorimetric methods to quan tify the tannin content, or to create reliable controls when using tannins in experimental studies. Insoluble polyvinylpolypyrrolidone (PVPP) can be u sed to specifically remove tannins from solution. In the present study, we evaluated the effect of different factors (amount of PVPP. number of PVPP t reatments, type of solvent, pH, and incubation time) on the PVPP removal of dissolved brown algal phlorotannins. Our results imply that there is a lim ited amount of phlorotannins that can bind to a given amount of PVPP. and t hat it is preferable to use low quantities of PVPP repeatedly, compared to using fewer treatments with a high amount of PVPP. Furthermore, we found no consistent effect on the removal of phlorotannins due to solvent type (ace tone, methanol, distilled water or filtered seawater). There was a slight d ecrease in the amount of phlorotannins removed from extracts with increasin g pH when repeatedly treated with PVPP. All phlorotannins were removed from extracts with pH less than or equal to 6.2, and 89% of the initial phlorot annin content was removed at pH 9.7. These results are compared with previo us methodological studies on tannin removal with PVPP. Furthermore. the imp lications of phlorotannin removal in analytical and ecological investigatio ns are discussed.