The inhibitory effects of colchicine on cell proliferation and mineralisation in culture

Citation
M. Salai et al., The inhibitory effects of colchicine on cell proliferation and mineralisation in culture, J BONE-BR V, 83B(6), 2001, pp. 912-915
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Ortopedics, Rehabilitation & Sport Medicine","da verificare
Journal title
JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME
ISSN journal
0301-620X → ACNP
Volume
83B
Issue
6
Year of publication
2001
Pages
912 - 915
Database
ISI
SICI code
0301-620X(200108)83B:6<912:TIEOCO>2.0.ZU;2-6
Abstract
Colchicine is often used in the treatment of diseases such as familial Medi terranean fever (FMF) and gout. We have previously reported that patients w ith FMF who had colchicine on a daily basis and who had a total hip arthrop lasty showed no heterotopic ossification after surgery. The mechanism by wh ich colchicine causes this clinical phenomenon has never been elucidated. W e therefore evaluated the effect of various concentrations of colchicine on cell proliferation and mineralisation in tissue culture, using rat and hum an cells with and without osteogenic potential. Cell proliferation was asse ssed by direct cell counts and uptake of (H-3)thymidine, and mineralisation by measuring the amount of staining by Alizarin Red. Our findings indicate that concentrations of colchicine of up to 3 ng/ml di d not affect cell proliferation but inhibition was observed at 10 to 30 ng/ mL Mineralisation decreased to almost 50%, which was the maximum inhibition observed, at concentrations of colchicine of 2.5 ng/ml. These results indi cate that colchicine at low concentrations, of up to 3 ng/ml, has the capac ity to inhibit selectively bone-like cell mineralisation in culture, withou t affecting cell proliferation. Further clinical and laboratory studies are necessary to evaluate the effects of colchicine on biological processes in volving the proliferation of osteoblasts and tissue mineralisation in vivo, such as the healing of fractures, the formation of heterotopic bone and ne oplastic bone growth.