Identification and molecular cloning of a novel brain-specific receptor protein that binds to brain injury-derived neurotrophic peptide - Possible role for neuronal survival

Citation
T. Hama et al., Identification and molecular cloning of a novel brain-specific receptor protein that binds to brain injury-derived neurotrophic peptide - Possible role for neuronal survival, J BIOL CHEM, 276(34), 2001, pp. 31929-31935
Citations number
42
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
0021-9258 → ACNP
Volume
276
Issue
34
Year of publication
2001
Pages
31929 - 31935
Database
ISI
SICI code
0021-9258(20010824)276:34<31929:IAMCOA>2.0.ZU;2-P
Abstract
Brain injury-derived neurotrophic peptide (BIN-P) is a synthetic 13-mer pep tide that supports neuronal survival and protects hippocampal neurons in pr imary cultures from cell death caused by glutamate. We have developed a mon oclonal antibody named mAb 6A22 against the 40-kDa BINP-binding protein, p4 0BBP. mAb 6A22 inhibits binding between BINP and rat brain synaptosomes and abolishes the protective effect of BINP. The antigen of mAb 6A22 should be the BINP-binding protein that mediates the neuroprotective action of BINP. Using an expression cloning approach with mAb 6A22, we isolated a cDNA enc oding a novel receptor protein that shows binding activity of BINP. COS7 ce lls transfected with the cloned cDNA show binding of BIN-P and cell surface s that are stained by 6A22. The mRNA for p40BBP is specific for the rat bra in and is increased after birth. From immunohistochemical studies using mAb 6A22, p40BBP increased after kainic acid treatment in rat hippocampal neur ons.