Lipopolysaccharide induction of indoleamine 2,3-dioxygenase is mediated dominantly by an IFN-gamma-independent mechanism

Citation
S. Fujigaki et al., Lipopolysaccharide induction of indoleamine 2,3-dioxygenase is mediated dominantly by an IFN-gamma-independent mechanism, EUR J IMMUN, 31(8), 2001, pp. 2313-2318
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
EUROPEAN JOURNAL OF IMMUNOLOGY
ISSN journal
0014-2980 → ACNP
Volume
31
Issue
8
Year of publication
2001
Pages
2313 - 2318
Database
ISI
SICI code
0014-2980(200108)31:8<2313:LIOI2I>2.0.ZU;2-9
Abstract
Indoleamine 2,3-dioxygenase (IDO) is a rate-limiting enzyme in the L-trypto phan-kynurenine pathway, which converts an essential amino acid, L-tryptoph an, to N-formylkynurenine. It has been speculated that IFN-gamma is a domin ant IDO inducer in vivo. The present study used IFN-gamma or TNF-alpha gene -disrupted mice and IFN-gamma anti body-treated mice to demonstrate that li popolysaccharicle (LPS)-induced systemic IDO is largely dependent on TNF-al pha rather than IFN-gamma. IFN-gamma -independent IDO induction was also de monstrated in vitro with LPS-stimulated monocytic THP-1 cells. These findin gs clearly indicate that there is an IFN-gamma -independent mechanism of ID O induction in addition to the IFN-gamma -dependent mechanism.