C-60 was covalently attached onto a self-assembly monolayer (SAM) of glutat
hione (GSH) formed on a Au electrode. Characterization of the resulting mon
olayer was carried out on a FT-IR spectrometer and its electrochemical prop
erties were investigated with cyclic voltammetry and impedance methods. It
was found that the pinholes on the C-60-GSH monolayer showed the response o
f the opening and closing with the change of pH. The modified electrode can
be used to determine NADH in neutral solution.