Mass spectrometric high-throughput analysis of serum-free conditioned medium from cancer cell lines

Citation
K. Sato et al., Mass spectrometric high-throughput analysis of serum-free conditioned medium from cancer cell lines, CANCER LETT, 170(2), 2001, pp. 153-159
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
CANCER LETTERS
ISSN journal
0304-3835 → ACNP
Volume
170
Issue
2
Year of publication
2001
Pages
153 - 159
Database
ISI
SICI code
0304-3835(20010920)170:2<153:MSHAOS>2.0.ZU;2-M
Abstract
Proteins secreted by cancer cells may be useful as tumor markers. We analyz ed constituents produced by cells grown in serum-free conditioned medium to avoid confounding factors present in serum. Mass spectrometric techniques were used to obtain expression patterns of constituents between 2000 and 15 000 Da from as little as I [il of crude sample. This protocol can produce distinctive mass profiles from 16 cell lines within 1 h. Thus, differential display by mass spectrometry will expedite the discovery of peptides speci fically secreted by particular cells. These data illustrate the advantage o f this strategy over the conventional approach of electrophoretic separatio n of serum samples. (C) 2001 Elsevier Science Ireland Ltd. All rights reser ved.