The N-terminal internal region of BLM is required for the formation of dots/rod-like structures which are associated with SUMO-1

Citation
H. Suzuki et al., The N-terminal internal region of BLM is required for the formation of dots/rod-like structures which are associated with SUMO-1, BIOC BIOP R, 286(2), 2001, pp. 322-327
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
286
Issue
2
Year of publication
2001
Pages
322 - 327
Database
ISI
SICI code
0006-291X(20010817)286:2<322:TNIROB>2.0.ZU;2-F
Abstract
Bloom Syndrome (BS) is a human autosomal genetic disorder characterized by a predisposition to a variety of malignant tumors. The gene responsible for BS encodes a protein (BLM) consisting of 1417 amino acids with a nuclear l ocalization signal in the C-terminal region, which is a member of the RecQ helicase family. We previously showed, using a yeast two-hybrid system, tha t BLM interacted with Ubc9, which is the conjugating enzyme of SUMO-1 (smal l ubiquitin-related modifier-1). In the present study, we exogenously expre ssed a green fluorescent protein-tagged Bloom syndrome protein, GFP-BLM, in human 293EBNA cells and found that it formed dots/rod-like structures asso ciated with SUMO-1 in the nucleus. Deletion experiments indicated that the region from amino acids 238 to 586 of BLM is required for the formation of dots/rod-like structures associated with SUMO-1, and the DNA helicase domai n, but not the helicase activity itself, slightly affected the formation an d/or stability of these structures. Expression of a GFP-BLM which contained the 238-586 region, but lacked the C-terminal nuclear localization signal, resulted in localization to the cytoplasm without the formation of dots/ro d-like structures and association with SUMO-1, indicating that these events occur only in the nucleus. (C) 2001 Academic Press.