Transformation of 2,2 '-dichlorodiisopropyl ether in mixed and pure culture

Citation
R. Hauck et al., Transformation of 2,2 '-dichlorodiisopropyl ether in mixed and pure culture, APPL MICR B, 56(3-4), 2001, pp. 491-495
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN journal
0175-7598 → ACNP
Volume
56
Issue
3-4
Year of publication
2001
Pages
491 - 495
Database
ISI
SICI code
0175-7598(200108)56:3-4<491:TO2'EI>2.0.ZU;2-I
Abstract
An aerobic enrichment culture derived from a groundwater contaminated with organic and chloroorganic compounds was adapted to the transformation of 2, 2 ' -dichlorodiisopropyl ether (DDE) in a continuous fixed-bed bioreactor. Continuous DDE removal efficiencies over 90% were achieved with a model wat er containing 3.3 mM methanol as co-substrate at DDE loading rates of up to 150 pmol l(-1) day(-1) with a hydraulic retention time of 24 h. In batch c ultures, a stoichiometric release of 2 mu mol chloride per pmol DDE transfo rmed was observed. From the mixed culture, a strain was isolated that is ab le to grow on DDE as the sole energy and carbon source, tolerating DDE conc entrations of up to 1 mM. Based on 16S rRNA sequencing, the strain is affil iated with the genus Rhodococcus.