An aerobic enrichment culture derived from a groundwater contaminated with
organic and chloroorganic compounds was adapted to the transformation of 2,
2 ' -dichlorodiisopropyl ether (DDE) in a continuous fixed-bed bioreactor.
Continuous DDE removal efficiencies over 90% were achieved with a model wat
er containing 3.3 mM methanol as co-substrate at DDE loading rates of up to
150 pmol l(-1) day(-1) with a hydraulic retention time of 24 h. In batch c
ultures, a stoichiometric release of 2 mu mol chloride per pmol DDE transfo
rmed was observed. From the mixed culture, a strain was isolated that is ab
le to grow on DDE as the sole energy and carbon source, tolerating DDE conc
entrations of up to 1 mM. Based on 16S rRNA sequencing, the strain is affil
iated with the genus Rhodococcus.