Isolation and characterization of a bacterium that produces hydrocarbons extracellularly which are equivalent to light oil

Citation
Mo. Park et al., Isolation and characterization of a bacterium that produces hydrocarbons extracellularly which are equivalent to light oil, APPL MICR B, 56(3-4), 2001, pp. 448-452
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN journal
0175-7598 → ACNP
Volume
56
Issue
3-4
Year of publication
2001
Pages
448 - 452
Database
ISI
SICI code
0175-7598(200108)56:3-4<448:IACOAB>2.0.ZU;2-M
Abstract
A halotorelant bacterial strain that produces a significant amount of lipid s from short-chain fatty acids was isolated from the sludge of a sewage dis posal plant. This strain displayed a significant extracellular accumulation of lipids. The yield of lipids including hydrocarbons was highest (120% of cell dry weight) at the end of the linear growth phase. Fractionation of t he lipids using thin-layer chromatography and subsequent gas chromatography showed that hydrocarbons were also obtained following an increase in total lipids. Their yield was the highest (50% of cell dry weight) in the linear growth phase. Additional analysis using infrared absorption spectrum and g as chromatography-mass spectrometry showed that the hydrocarbon fraction wa s composed of alkanes, such as C15H32, C18H38, C21H44, C22H46 and C24H50. H omology analysis of the 16s rDNA sequence as well as studies of the morphol ogical and physiological characteristics indicated that the bacterium is a strain of Vibrio furnissii.