An enzyme-linked immunosorbent assay (ELISA) using a polyclonal antibody ag
ainst octa-chlorinated dibenzo-p-dioxin (OCDD) is presented. This method is
based on a competitive reaction between OCDD and OCDD-HRP (horseradish per
oxidase) conjugate against the antibody, whereby OCDD-HRP is detected color
imetrically at 450 nm. The detection limit of OCDD was 0.78 mug mL(-1). Opt
imizing the reaction conditions of the assay, cross reactivities of some di
oxins against the antibody are discussed.