Validity and safety of sputum induction by inhaled uridine 5 '-triphosphate

Citation
J. Tamaoki et al., Validity and safety of sputum induction by inhaled uridine 5 '-triphosphate, AM J R CRIT, 164(3), 2001, pp. 378-381
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cardiovascular & Respiratory Systems","da verificare
Journal title
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
ISSN journal
1073-449X → ACNP
Volume
164
Issue
3
Year of publication
2001
Pages
378 - 381
Database
ISI
SICI code
1073-449X(20010801)164:3<378:VASOSI>2.0.ZU;2-6
Abstract
Inhalation of hypertonic saline during sputum induction causes bronchoconst riction. We studied the validity and safety of sputum induction by uricline 5 ' -triphosphate (UTP). Sputum was induced by a 5-min inhalation of hyper tonic saline (3%) on Day 1 and UTP (5 mg/ml in 0.9% saline) on Days 8 and 1 5 in 16 healthy subjects and 16 patients with mild-to-moderate asthma. Inha led UTP produced twofold greater amounts of sputum than did hypertonic sali ne. There were significant differences in oxygen desaturation and bronchoco nstriction during the procedure between the two methods: the maximal fall i n Sa(O2), the AUC of the Sa(O2)-time response, and the fall in PEF were les s in the subjects who received UTP than in those who received hypertonic sa line. Sputum total cell and differential cell counts, with a high proportio n of eosinophils in asthmatics, were similar between specimens obtained by hypertonic saline and UTP. When we compared two consecutive measurements on the UTP-induced sputum samples, the reproducibility calculated by the intr aclass correlation coefficient was high for the proportion of eosinophils, neutrophils, and macrophages. Therefore, inhalation of UTP aerosols may pro vide an effective, relatively noninvasive, valid, and reproducible method o f sputum induction for the assessment of airway inflammation in asthma.