Induction of transcription factor AP-2 by cytokines and prostaglandins in cultured mesangial cells

Citation
K. Suyama et al., Induction of transcription factor AP-2 by cytokines and prostaglandins in cultured mesangial cells, AM J NEPHR, 21(4), 2001, pp. 307-314
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Urology & Nephrology
Journal title
AMERICAN JOURNAL OF NEPHROLOGY
ISSN journal
0250-8095 → ACNP
Volume
21
Issue
4
Year of publication
2001
Pages
307 - 314
Database
ISI
SICI code
0250-8095(200107/08)21:4<307:IOTFAB>2.0.ZU;2-C
Abstract
Background/aims: Activator protein-2 (AP-2) is an important transcription f actor for activation of growth- and inflammatory-associated genes. To detec t AP-2 in the mesangium, the expression level of AP-2 was examined in cultu red mesangial cells in response to various cytokines and prostaglandins. Th e level was also observed in kidney tissue samples obtained from patients w ith proteinuria and from a rat nephrosis model. Methods: AP-2 was immunohis tochemically detected with a specific antibody. The expression level was an alyzed by immunoblotting. Human tissue samples were obtained from patients with proteinuria. Kidney samples were also obtained from rats with puromyci n aminonucleoside-induced nephrosis. Results: Pro-inflammatory cytokines, s uch as IL-6, IL-1 and IL-2, but not TNF-alpha, induced AP-2 expression in a time- and dose-dependent manner in cultured mesangial cells. PGE(2) and PG I(2) also induced AP-2 expression, while PGF(2 alpha) failed to induce this protein. High expression levels of AP-2 were observed in different cell ty pes including mesangial cells of kidney samples from patients with proteinu ria. Similar results were obtained from the rat nephrosis model. Conclusion : These findings demonstrate that the primary cytokines induce AP-2 protein in mesangial cells. AP-2 may act as a transcription factor to produce addi tional cytokines and growth-associated gene products, suggesting an importa nt role for AP-2 for the function of mesangial cells in glomerular disorder s. Copyright (C) 2001 S. Karger AG, Basel.