Multiphoton confocal microscopy using a femtosecond Cr : forsterite laser

Citation
Tm. Liu et al., Multiphoton confocal microscopy using a femtosecond Cr : forsterite laser, SCANNING, 23(4), 2001, pp. 249-254
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary,"Spectroscopy /Instrumentation/Analytical Sciences
Journal title
SCANNING
ISSN journal
0161-0457 → ACNP
Volume
23
Issue
4
Year of publication
2001
Pages
249 - 254
Database
ISI
SICI code
0161-0457(200107/08)23:4<249:MCMUAF>2.0.ZU;2-7
Abstract
With its output wavelength covering the infrared penetrating window of most biological tissues at 1200-1250 rim, the femtosecond Cr:forsterite laser s hows high potential to serve as an excellent excitation source for the mult iphoton fluorescence microscope. Its high output power, short optical pulse width., high stability, and low dispersion in fibers make it a perfect rep lacement for the currently widely used Ti:sapphire laser. In this paper, we study the capability of using a femtosecond Cr: forsterite laser in multip hoton scanning microscopy. We have performed the multiphoton excited photol uminescence spectrum measurement on several commonly used bioprobes using t he 1230 nm femtosecond pulses from a Cr:forsterite laser. Efficient fluores cence can be easily observed in these bioprobes through two-photon or three -photon excitation processes. These results will assist in the selection of dichroic beam splitter and band pass filters in a multiphoton microscopic system. We have also performed the autofluorescence spectrum measurement fr om chlorophylls in live leaves of the plant Arabidopsis thaliana excited by 1230 nm femtosecond pulses from the Cr:forsterite laser. Bright luminescen ce from chlorophyll, centered at 673 and 728 nm, respectively, can be easil y observed. Taking advantage of the bright two-photon photoluminescence fro m chlorophyll, we demonstrated the two-photon scanning paradermal and cross -sectional images of palisade mesophyll cells in live leaves of Arabidopsis thaliana.