A method for isolating a high yield of Arabidopsis chloroplasts capable ofefficient import of precursor proteins

Fitzpatrick, LM Keegstra, K

Lm. Fitzpatrick et K. Keegstra, A method for isolating a high yield of Arabidopsis chloroplasts capable ofefficient import of precursor proteins, PLANT J, 27(1), 2001, pp. 59-65

22

INGLESE

Article

Plant Sciences","Animal & Plant Sciences

PLANT JOURNAL

0960-7412 → ACNP

27

1

2001

59 - 65

ISI

0960-7412(200107)27:1<59:AMFIAH>2.0.ZU;2-#

Chloroplasts were isolated from Arabidopsis plants grown under different co nditions, and using different protocols, to determine a method that would y ield chloroplasts capable of binding and importing precursor proteins. Chlo roplasts isolated from protoplasts and purified on a Percoll gradient were highly import-competent, with little non-specific binding of the precursor, and a high yield of intact chloroplasts (0.1 mg chlorophyll/g FW). Chlorop lasts from plants grown on agar plates had a much higher rate of import tha n those from plants grown on soil. Protein import remained high at all of t he ages tested for chloroplasts from plate-grown plants, whereas it decline d during the development of soil-grown plants. Arabidopsis chloroplasts imp orted a range of precursor proteins and had nucleotide requirements for bin ding and import similar to those reported for pea chloroplasts.