A method for isolating a high yield of Arabidopsis chloroplasts capable ofefficient import of precursor proteins

Citation
Lm. Fitzpatrick et K. Keegstra, A method for isolating a high yield of Arabidopsis chloroplasts capable ofefficient import of precursor proteins, PLANT J, 27(1), 2001, pp. 59-65
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT JOURNAL
ISSN journal
0960-7412 → ACNP
Volume
27
Issue
1
Year of publication
2001
Pages
59 - 65
Database
ISI
SICI code
0960-7412(200107)27:1<59:AMFIAH>2.0.ZU;2-#
Abstract
Chloroplasts were isolated from Arabidopsis plants grown under different co nditions, and using different protocols, to determine a method that would y ield chloroplasts capable of binding and importing precursor proteins. Chlo roplasts isolated from protoplasts and purified on a Percoll gradient were highly import-competent, with little non-specific binding of the precursor, and a high yield of intact chloroplasts (0.1 mg chlorophyll/g FW). Chlorop lasts from plants grown on agar plates had a much higher rate of import tha n those from plants grown on soil. Protein import remained high at all of t he ages tested for chloroplasts from plate-grown plants, whereas it decline d during the development of soil-grown plants. Arabidopsis chloroplasts imp orted a range of precursor proteins and had nucleotide requirements for bin ding and import similar to those reported for pea chloroplasts.