Effect of basement membrane components on assembly of 3-pyroglutamylamyloid beta-protein

Citation
Y. Kiuchi et al., Effect of basement membrane components on assembly of 3-pyroglutamylamyloid beta-protein, NEUROSC R C, 29(1), 2001, pp. 41-49
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROSCIENCE RESEARCH COMMUNICATIONS
ISSN journal
0893-6609 → ACNP
Volume
29
Issue
1
Year of publication
2001
Pages
41 - 49
Database
ISI
SICI code
0893-6609(200107/08)29:1<41:EOBMCO>2.0.ZU;2-X
Abstract
3-pyroglutamyl amyloid beta -protein is major component of senile plaques i n Alzheimer's disease. Basement membrane (BM) components co-localize with a myloid in the plaque. We investigated the potential of BM components, such as type IV collagen (collagen IV), laminin and entactin, to prevent assembl y of 3-pyroglutamyl amyloid beta -protein 3-42 (A beta 3pE-42) and to induc e a disassembly of A beta 3pE-42 fibril. For this we used thioflavin T fluo rometric assays. All BM components significantly inhibited A beta 3pE-42 fi bril formation (Molar ratio; A beta :collagen IV = 136:1, A beta :laminin = 180:1, A beta :entactin = 20:1). The inhibitory effect of BM components wa s in a dose-dependent manner. Collagen IV and laminin significantly disrupt ed pre-formed A beta pE-42 fibril, whereas entactin without effect. We prop ose that BM components may be involved in simile plaque formation by regula ting both A beta 3pE-42 fibril formation and disassembly of the fibril.