The core of the motor domain determines the direction of myosin movement

Citation
K. Homma et al., The core of the motor domain determines the direction of myosin movement, NATURE, 412(6849), 2001, pp. 831-834
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary,Multidisciplinary,Multidisciplinary
Journal title
NATURE
ISSN journal
0028-0836 → ACNP
Volume
412
Issue
6849
Year of publication
2001
Pages
831 - 834
Database
ISI
SICI code
0028-0836(20010823)412:6849<831:TCOTMD>2.0.ZU;2-N
Abstract
Myosins constitute a superfamily of at least 18 known classes of molecular motors that move along actin filaments(1-3). Myosins move towards the plus end of F-actin filaments; however, it was shown recently that a certain cla ss of myosin, class VI myosin, moves towards the opposite end of F-actin, t hat is, in the minus direction(4). As there is a large, unique insertion in the myosin VI head domain between the motor domain and the light-chain-bin ding domain (the lever arm), it was thought that this insertion alters the angle of the lever-arm switch movement, thereby changing the direction of m otility(4). Here we determine the direction of motility of chimaeric myosin s that comprise the motor domain and the lever-arm domain (containing an in sert) from myosins that have movement in the opposite direction. The result s show that the motor core domain, but neither the large insert nor the con verter domain, determines the direction of myosin motility.