Synergistic effect of cadmium chloride and acetaldehyde on cytotoxicity and its prevention by quercetin and glycyrrhizin

Citation
Cc. Hu et al., Synergistic effect of cadmium chloride and acetaldehyde on cytotoxicity and its prevention by quercetin and glycyrrhizin, MUT RES-GTE, 496(1-2), 2001, pp. 117-127
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
ISSN journal
1383-5718 → ACNP
Volume
496
Issue
1-2
Year of publication
2001
Pages
117 - 127
Database
ISI
SICI code
1383-5718(20010920)496:1-2<117:SEOCCA>2.0.ZU;2-0
Abstract
Cadmium chloride at concentrations of 10-50 mM and acetaldehyde (AA) at 1-5 mM showed synergistic toxic effects on V79 cells in vitro. Furthermore, sy nergistic effects of these chemicals were also observed in mutagenicities o f the Hprt gene within certain dose ranges (cadmium chloride 5-10mM, and AA 1-2.5 mM). Moreover, lipid peroxide formation, malondialdehyde (MDA) forma tion, detected by 2-thiobarbituric acid (TBA) reaction and the mitochondria l membrane potentials detected by rhodamine 123 uptake were significantly i ncreased with the combined effect of cadmium and AA in V79. Thus, the cytot oxicity and genotoxicity displayed by combination of these chemicals can be considered to be associated with oxidative stress. Further, these effects were efficiently reduced by quercetin and less efficiently with glycyrrhizi n. (C) 2001 Elsevier Science B.V. All rights reserved.