The superiority of organically cultivated vegetables to general ones regarding antimutagenic activities

Citation
Hf. Ren et al., The superiority of organically cultivated vegetables to general ones regarding antimutagenic activities, MUT RES-GTE, 496(1-2), 2001, pp. 83-88
Citations number
5
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
ISSN journal
1383-5718 → ACNP
Volume
496
Issue
1-2
Year of publication
2001
Pages
83 - 88
Database
ISI
SICI code
1383-5718(20010920)496:1-2<83:TSOOCV>2.0.ZU;2-N
Abstract
We found organically cultivated (OC) vegetables, using a water-soluble chit osan as a soil improvement agent and leaf surface spray, had much longer sh elf life and better taste than that of generally cultivated (GC) vegetables . The purpose of this study is to determine the relative antimutagenic acti vity between OC and GC vegetables. Eleven OC vegetables were harvested in M arch and April in 1999 and 2000, and GC ones were supplied as a control fro m nearby farms on the same date. The former vegetables were planted on the field where no pesticide had been used for the last 3 years. Forward mutati on test with Salmonella typhimurium TM677 and 8-azaguanine as a detection a gent was used to determine the antimutagenic activity of juices prepared fr om OC and GC vegetables against authentic mutagens, such as 4-nitroquinolin e oxide (4NQO), benzo(a)pyrene (BaP), and 3-amino-1-methyl-5H-pyrido[4,3-b] indole acetate (Trp-P-2). This microbiological test is a convenient method to use for the food samples containing free histidine. Antimutagenic activi ty was evaluated by the difference of mutagenic activities between mutagene city of authentic compounds and that observed upon incubation at 37 degrees C for 2 h with each vegetable juice. OC Chinese cabbage, carrot, Welsh onio n, and Qing-gen-cai suppressed 37-93% of the mutagenic activity of 4NQO, wh ile the GC ones were held down to 11-65%. Against BaP, three species of OC vegetables showed 30-57% antimutagenecity, while GC ones did only 5-30%. Si milarly, the OC spinach decreased the activity of Trp-P-2 to 78%, and the G C suppressed it by 49%. (C) 2001 Elsevier Science B.V. All rights reserved.