Protection against ultraviolet B- and C-induced DNA damage and skin carcinogenesis by the flowers of Prunus persica extract

Citation
My. Heo et al., Protection against ultraviolet B- and C-induced DNA damage and skin carcinogenesis by the flowers of Prunus persica extract, MUT RES-GTE, 496(1-2), 2001, pp. 47-59
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
ISSN journal
1383-5718 → ACNP
Volume
496
Issue
1-2
Year of publication
2001
Pages
47 - 59
Database
ISI
SICI code
1383-5718(20010920)496:1-2<47:PAUBAC>2.0.ZU;2-X
Abstract
The ethanol extract of the flowers of Prunus persica (Ku-35) (50-200 mug/ml ) was found to inhibit UVB- as well as UVC-induced DNA damage measured by t he COMET assay in the skin fibroblast cell (NIH/3T3). In addition, Ku-35 in hibited UVB- or UVC-induced lipid peroxidation, especially against UVB-indu ced peroxidation at higher than 10 mug/ml. We also evaluated the protective effect of Ku-35 against UVB-induced non-melanoma skin cancer in mice. Ku-3 5 was applied topically before UVB exposure, and its effects on tumor incid ence (% of mice with tumors) and tumor multiplicity (number of tumors per m ouse) were evaluated. The application of Ku-35 clearly resulted in a delay of tumor development compared to the control. In tumor incidence, 100% mice in the control group and the low dose treatment of Ku-35 had tumors, where as 94.1% of the mice had tumors after the high dose treatment of Ku-35 at t he end of experiment (28 weeks). In tumor multiplicity, low and high treatm ents of Ku-35 resulted in 25.9 and 53.9% reduction at the end of the experi ment (P < 0.05, one-way analysis of variance (ANOVA)). The present data ind icate that Ku-35 protects against photogencitoxicity in NIH/3T3 fibroblasts . The possible action mechanism of Ku-35 may be through its anti-oxidant ac tivity without pro-oxidant effect. Ku-35 can also show a delay of tumor dev elopment against UVB-induced skin carcinogenesis. These results suggest tha t Ku-35 extract may be useful for protecting UV-induced DNA damage and carc inogenesis when topically applied. (C) 2001 Elsevier Science B.V. All right s reserved.