Immunological detection of a CK2 activity in Tapes semidecussatus (Mollusca, Bivalvia) mantle

Citation
L. Mercado et al., Immunological detection of a CK2 activity in Tapes semidecussatus (Mollusca, Bivalvia) mantle, MALACOLOGIA, 43(1-2), 2001, pp. 217-222
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Animal Sciences
Journal title
MALACOLOGIA
ISSN journal
0076-2997 → ACNP
Volume
43
Issue
1-2
Year of publication
2001
Pages
217 - 222
Database
ISI
SICI code
0076-2997(2001)43:1-2<217:IDOACA>2.0.ZU;2-S
Abstract
Casein kinase 2 (CK2) is a highly conserved enzyme present in a wide range of eukaryotes, including yeasts, invertebrates and mammals. This kinase is involved in a number of key regulatory cellular processes. We describe a CK 2-like protein in mantle tissue of Tapes semidecussatus, a highly active or gan responsible for cell healing and regeneration in this clam species. Aft er subcellular fractionation of crude homogenized mantles, the putative enz ymatic activity was clearly enriched after two-step cromatography partial p urification. Furthermore, using a polyclonal antibody elicited against the known subunits of the mammalian enzyme, Western blot analysis confirmed the presence of three reactive polypeptides. Two of these perfectly matched th e expected molecular weights of the catalytic alpha (42 kDa) and alpha ' (3 8 kDa) subunits of the reference enzyme. Considering our research interest is centered on the relationship between marine organisms and their environm ent, our preliminary work on a CK2-like kinase in clams offers a potential model system to study protein phosphorylation in molluscs as an indicator o f the effect of environment factors over selected physiological responses.