Biphasic response of NK cells expressing both activating and inhibitory killer Ig-like receptors

Citation
Hs. Warren et al., Biphasic response of NK cells expressing both activating and inhibitory killer Ig-like receptors, INT IMMUNOL, 13(8), 2001, pp. 1043-1052
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
INTERNATIONAL IMMUNOLOGY
ISSN journal
0953-8178 → ACNP
Volume
13
Issue
8
Year of publication
2001
Pages
1043 - 1052
Database
ISI
SICI code
0953-8178(200108)13:8<1043:BRONCE>2.0.ZU;2-L
Abstract
NK cells can co-express inhibitory and activating killer Ig-like receptors (KIR) recognizing the same HLA class I ligand. We present evidence from exp eriments with NK cells expressing both activating (KIR2DS2) and inhibitory (KIR2DL2 and KIR2DL3) receptors that the activating KIR can function withou t apparent interference from the inhibitory KIR. These studies used CD158b mAb that is equally reactive with KIR2DS2, KIR2DL2 and KIR2DL3. First, we s how using plastic-immobilized CD158b mAb that the activating KIR2DS2 is sti mulated, resulting in NK cell division and degranulation. Second, we show u sing soluble CD158b mAb and FcRII(+) P815 cells that high concentrations of CD158b mAb trigger the inhibitory KIR, whereas low concentrations stimulat e the activating KIR2DS2 resulting in NK cell division and cytolysis. These results demonstrate that the activating KIR2DS2 can function on cells co-e xpressing the inhibitory KIR2DL2 and/or KIR2DL3, indicating the potential f or independent function of activating KIR with natural ligand.