RNA editing in higher plant plastids: oligoribonucleotide SSCP analysis allows the proof of base conversion directly at the RNA level

Citation
M. Fuchs et al., RNA editing in higher plant plastids: oligoribonucleotide SSCP analysis allows the proof of base conversion directly at the RNA level, CURR GENET, 39(5-6), 2001, pp. 384-387
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
CURRENT GENETICS
ISSN journal
0172-8083 → ACNP
Volume
39
Issue
5-6
Year of publication
2001
Pages
384 - 387
Database
ISI
SICI code
0172-8083(200107)39:5-6<384:REIHPP>2.0.ZU;2-A
Abstract
Plastid RNA editing of a number of transcripts at specific sites changes ge nomically encoded cytidines to nucleosides, which act like uridines in RT-P CR analyses. To study plastid-editing directly at the RNA level, we establi shed a single-strand conformational polymorphism, assay for the discriminat ion of small RNA molecules. The electrophoretic mobility of a oligoribonucl eotide resulting from a RNase TI-digested and edited plastid mRNA was shown to be identical with a control RNA molecule containing a uridine at the ed iting, site, whereas the unedited RNA behaved like a RNA molecule containin g a cytidine at the respective position.