De-novo humoral immune responses to cancer-associated autoantigens during transition from chronic liver disease to hepatocellular carcinoma

Citation
Jy. Zhang et al., De-novo humoral immune responses to cancer-associated autoantigens during transition from chronic liver disease to hepatocellular carcinoma, CLIN EXP IM, 125(1), 2001, pp. 3-9
Citations number
55
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
ISSN journal
0009-9104 → ACNP
Volume
125
Issue
1
Year of publication
2001
Pages
3 - 9
Database
ISI
SICI code
0009-9104(200107)125:1<3:DHIRTC>2.0.ZU;2-Z
Abstract
A feature of hepatocellular carcinoma (HCC) is that antecedent liver cirrho sis and chronic hepatitis are common precursor conditions and during transi tion to malignancy some patients develop autoantibodies which were not pres ent during the preceding chronic liver disease phase. Serum samples from su ch patients can be used to immunoscreen cDNA expression libraries to identi fy genes encoding the new autoantigens. We demonstrate here the de novo app earance of antibodies to p62, a cytoplasmic protein which has been shown to bind to a developmentally regulated fetal species of insulin-like growth f actor II (IGF-II) mRNA. Another antibody appearing during the transition pe riod was against CENP-F, a cell cycle-related nuclear protein with maximum expression in the G2 and M phases of the cell cycle and previously shown to have a high association with malignancy. In three additional patients in w hom serial serum samples were examined, new appearance of anti-p62 was dete cted in two patients and anti-CENP-F in one patient. This study demonstrate s that transition to malignancy can be associated with autoantibody respons es to certain cellular proteins which might have some role in tumorigenesis .