Water-soluble and water-insoluble glucans produced by Escherichia coli recombinant dextransucrases from Leuconostoc mesenteroides NRRL B-512F

Citation
K. Funane et al., Water-soluble and water-insoluble glucans produced by Escherichia coli recombinant dextransucrases from Leuconostoc mesenteroides NRRL B-512F, CARBOHY RES, 334(1), 2001, pp. 19-25
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
CARBOHYDRATE RESEARCH
ISSN journal
0008-6215 → ACNP
Volume
334
Issue
1
Year of publication
2001
Pages
19 - 25
Database
ISI
SICI code
0008-6215(20010803)334:1<19:WAWGPB>2.0.ZU;2-S
Abstract
Two dextransucrase genes, dsrS and dsrT5, from Leuconostoc mesenteroides NR RL B-512F were expressed in Escherichia coli, and recombinant dsrT5 dextran sucrase was shown to produce a water-insoluble glucan. In contrast, native dextran from L. mesenteroides B-512F is water-soluble. The water-insoluble glucan was shown by C-13 NMR and glycosyl-linkage composition analysis to c ontain about 50% 6-linked Glcp and 40% 3-linked Glcp. The 'primitive' B-512 F strain is suggested to have produced water-insoluble glucan containing 3- linked Glcp. The glucans produced by dextransucrases expressed in E. coli c ontained 4-linked Glcp, as shown by glycosyl-linkage composition analysis. The amount of 4-linked Glcp was increased when the truncated, water-insolub le, glucan-producing dextransucrase, which does not have C-terminal repeati ng units, was added to the water-soluble, glucan-producing dextransucrase. Trace amounts of 4-linked Glcp were also detected in the dextran obtained f rom the B-512F culture supernatant, in dextran produced by dextransucrase p urified from the B-512F strain culture supernatant, and in clinical dextran . The results of glycosyl-linkage composition analysis suggest that dextran sucrases produce 4-linked Glcp as well as 6- and 3-linked Glcp. (C) 2001 Pu blished by Elsevier Science Ltd.