Generation of calcium waves in living cells by pulsed-laser-induced photodisruption

Citation
Ni. Smith et al., Generation of calcium waves in living cells by pulsed-laser-induced photodisruption, APPL PHYS L, 79(8), 2001, pp. 1208-1210
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science
Journal title
APPLIED PHYSICS LETTERS
ISSN journal
0003-6951 → ACNP
Volume
79
Issue
8
Year of publication
2001
Pages
1208 - 1210
Database
ISI
SICI code
0003-6951(20010820)79:8<1208:GOCWIL>2.0.ZU;2-K
Abstract
Here, we present an optical technique that can induce waves of calcium ion concentration in live biological cells. Ca2+ waves were induced by femtosec ond pulsed-laser illumination. Living HeLa cells were exposed to focused 14 0 fs pulses of 780 nm wavelength at 30 mW average power. Ca2+ waves were im aged by fluorescence and were observed to propagate from the laser focal po int inside the cell. Photoinduced generation of Ca2+ waves can be performed at any point inside the cell, an improvement over previous mechanical or b iochemical stimulation techniques. (C) 2001 American Institute of Physics.