APC and K-ras gene mutation in aberrant crypt foci of human colon

Citation
P. Yuan et al., APC and K-ras gene mutation in aberrant crypt foci of human colon, WORLD J GAS, 7(3), 2001, pp. 352-356
Citations number
52
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Gastroenerology and Hepatology
Journal title
WORLD JOURNAL OF GASTROENTEROLOGY
ISSN journal
1007-9327 → ACNP
Volume
7
Issue
3
Year of publication
2001
Pages
352 - 356
Database
ISI
SICI code
1007-9327(200106)7:3<352:AAKGMI>2.0.ZU;2-B
Abstract
AIM To study the genetic alteration in ACF and to define the possibility th at ACF may be a very early morphological lesion with molecular changes. and to explore the relationship between ACF and colorectal adenoma even carcin oma. METHODS DNA from 35 CRC, 15 adenomas, 34 ACF and 10 normal mucus was isolat ed by means of microdissection. Direct gene sequencing of Kras gene includi ng codon 12, 13 and 61 as well as the mutation cluster region (MCR) of APC gene was performed. RESULTS K-ras gene mutation frequency in ACF, adenoma and carcinoma was 17. 6% (6/ 34), 13.3% ( 2/ 15), and 14.3% ( 5/ 35) respectively, showing no dif ference ( P > 0. 05) in K-ras gene mutation among three pathologic procedur es. The K-ras gene mutation in adenoma. carcinoma and 4 ACF restricted in c odon 12 (GGT --> GAT), but the other 2 mutations from ACF located in codon 13 (GGC --> GAC). Kras gene mutation was found more frequently in older pat ients and patients with polypoid cancer. No mutation in codon 61 was found in the three tissue types. Mutation rate of APC gene in adenoma and carcino ma was 22.9% (8/ 35) and 26.7% (4/ 15), which was higher than ACF (2.9%) ( P < 0.05). APC gene mutation in carcinoma was not correlated with age of pa tients. location, size and differentiation of tumor. CONCLUSION ACF might be a very early morphological lesion in the tumorogene sis of colorectal tumor. The morphological feature and gene mutation status was different in ACF and adenoma. ACF is possibly putative ". microadenoma " that might be the precursor of adenoma. In addition, the development of a subgroup of colorectal carcinomas might undergo a way of "normal epitheliu m --> ACF --> carcinomas".